SPHK1 antibody (AA 286-315)

Catalog No. ABIN3032710

This Rabbit Polyclonal antibody specifically detects SPHK1 in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Primate.

Quick Overview for SPHK1 antibody (AA 286-315) (ABIN3032710)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Human, Mouse, Rat, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-SPHK1 Antibody

Binding Specificity: AA 286-315

Purification: Purified

Immunogen: A portion of amino acids 286-315 from the human protein was used as the immunogen for this SPHK1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the SPHK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SPHK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: SPHK1

Background: Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

UniProt: Q9NYA1

Pathways: VEGF Signaling