SPHK1 antibody (AA 313-343)

Catalog No. ABIN3032708

The Rabbit Polyclonal anti-SPHK1 antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect SPHK1 in samples from Human.

Quick Overview for SPHK1 antibody (AA 313-343) (ABIN3032708)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-SPHK1 Antibody

Binding Specificity: AA 313-343

Purification: Purified

Immunogen: A portion of amino acids 313-343 from the human protein was used as the immunogen for this SPHK1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the SPHK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SPHK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: SPHK1

Background: SPHK1 is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.

UniProt: Q9NYA1

Pathways: VEGF Signaling