YAP1 antibody (AA 420-446)

Catalog No. ABIN3029661

Product Details anti-YAP1 Antibody

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Binding Specificity: AA 420-446

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purification: Purified

Immunogen: A portion of amino acids 420-446 from human YAP1 was used as the immunogen for this YAP antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the YAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the YAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Alternative Name: YAP (YAP1 Products)

Synonyms: YAP antibody, YAP2 antibody, YAP65 antibody, YKI antibody, xyap antibody, yap antibody, yap2 antibody, yap65 antibody, yki antibody, AI325207 antibody, Yap antibody, Yap65 antibody, Yki antibody, Yorkie antibody, cb194 antibody, sb:cb194 antibody, si:ch211-181p1.5 antibody, si:dkey-3b8.3 antibody, wu:fc18c04 antibody, zgc:158380 antibody, Yes associated protein 1 antibody, Yes associated protein 1 S homeolog antibody, yes-associated protein 1 antibody, Yes-associated protein 1 antibody, YAP1 antibody, yap1.S antibody, Yap1 antibody, yap1 antibody

Background: YAP is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. [UniProt]

UniProt: P46937

Pathways: MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha