AGO1 antibody (N-Term) (HRP)

Catalog No. ABIN4966093

This Rabbit Polyclonal antibody specifically detects AGO1 in WB. It exhibits reactivity toward Arabidopsis thaliana, Hyacinthus orientalis (Common hyacinth) and Nicotiana benthamiana.

Quick Overview for AGO1 antibody (N-Term) (HRP) (ABIN4966093)

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reactivity: Arabidopsis thaliana, Hyacinthus orientalis (Common hyacinth), Nicotiana benthamiana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO1 antibody is conjugated to HRP

Application: Western Blotting (WB)

Product Details anti-AGO1 Antibody (HRP)

Binding Specificity: N-Term

Purpose: Anti-AGO1 | Argonaute 1 (40 μg, HRP-conjugated)

Cross-Reactivity (Details): Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana benthamiana
Not reactive in: Chlamydomonas reinhardtii, Oryza sativa, Phaseolus vulgaris, Triticum aestivum, Zea mays

Predicted Reactivity: Brassica pekinensis,Capsella rubella,Glycine max,Malus domestica,Pisum sativum,Ricinus communis,Solanum tuberosum,Zea mays,Vitis vinifera

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379

Application Details

Application Notes: 1 : 1000 (WB)

Comment:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.

Handling Advice: A preservative may be added for long time storage

Storage: 4 °C

Storage Comment: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Expiry Date: 12-24 months

Target Details for AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Alternative Name: argonaute 1 (AGO1)

Background:

Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).This antibody is directly conjugated to Horseradish Peroxidase (HRP).

Additional information: Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.TCA acetone total protein precipitation method

Molecular Weight: 116.4 | 130 kDa

Gene ID: 841262

UniProt: O04379

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization