ATG3 antibody (Middle Region)

Catalog No. ABIN497989

This Rabbit Polyclonal antibody specifically detects ATG3 in WB and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ATG3 antibody (Middle Region) (ABIN497989)

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ATG3 Antibody

Binding Specificity: Middle Region

Purification: Immunoaffinity Chromatography

Immunogen: ATG3 antibody was raised against an 18 amino acid peptide near the center of Human ATG3 (GenBank accession no. NP_071933).

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: ATG3 antibody can be used for the detection of ATG3 at 1-2 μg/mL. Positive Control: Mouse Kidney Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for ATG3

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Alternative Name: APG3 / ATG3

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.Synonyms: APG3-like, APG3L, Autophagy-related protein 3, Protein PC3-96

Gene ID: 64422

NCBI Accession: NP_071933

UniProt: Q9NT62

Pathways: Autophagy