CSK antibody

Catalog No. ABIN498196

This Rabbit Polyclonal antibody specifically detects CSK in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CSK antibody (ABIN498196)

Target: CSK (C-Src tyrosine Kinase (CSK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CSK Antibody

Specificity: This antibody detects endogenous levels of Csk protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for CSK

Target: CSK (C-Src tyrosine Kinase (CSK))

Alternative Name: CSK

Background: C terminal Src kinase (CSK) is a non receptor protein tyrosine kinase. One of its major functions is to specifically phosphorylate a conserved C terminal tyrosine of Src family kinases thereby down regulating their kinase activity. CSK is expressed ubiquitously as a protein of approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft associated protein PAG (CSK binding protein).Synonyms: C-SRC kinase, Protein-tyrosine kinase CYL, Tyrosine-protein kinase CSK

Molecular Weight: approx. 50 kDa

Gene ID: 1445

NCBI Accession: NP_001120662

UniProt: P41240

Pathways: TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events