CSK antibody

Catalog No. ABIN498315

The Rabbit Polyclonal anti-CSK antibody (ABIN498315) specifically detects CSK in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CSK antibody (ABIN498315)

Target: CSK (C-Src tyrosine Kinase (CSK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CSK Antibody

Specificity: This antibody detects endogenous levels of Csk protein. (region surrounding asn63)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for CSK

Target: CSK (C-Src tyrosine Kinase (CSK))

Alternative Name: CSK

Background: C terminal Src kinase (CSK) is a non receptor protein tyrosine kinase. One of its major functions is to specifically phosphorylate a conserved C terminal tyrosine of Src family kinases thereby down regulating their kinase activity. CSK is expressed ubiquitously as a protein of approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft associated protein PAG (CSK binding protein).Synonyms: C-SRC kinase, Protein-tyrosine kinase CYL, Tyrosine-protein kinase CSK

Molecular Weight: approx. 50 kDa

Gene ID: 1445

NCBI Accession: NP_001120662

UniProt: P41240

Pathways: TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events