BHLHE41 antibody

Catalog No. ABIN498356

The Rabbit Polyclonal anti-BHLHE41 antibody is suitable to detect BHLHE41 in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for BHLHE41 antibody (ABIN498356)

Target: BHLHE41 (Basic Helix-Loop-Helix Family, Member E41 (BHLHE41))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BHLHE41 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BHLHE41 Antibody

Specificity: This antibody detects endogenous levels of DEC2 protein. (region surrounding Lys31)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: PBS, pH ~7.3, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BHLHE41

Target: BHLHE41 (Basic Helix-Loop-Helix Family, Member E41 (BHLHE41))

Alternative Name: BHLHB3 / BHLHE41

Background: Human DEC1 is a 412 amino acid, basic helix-loop-helix (bHLH) containing protein that is involved in the control of proliferation and/or differentiation of several cell types including nerve cells, fibroblasts and chondrocytes. The bHLH region of DEC1 is structurally similar to the bHLH regions of the mammalian HES family, Drosophila hairy, and Enhancer of split m7. DEC1 is a novel direct target for cAMP in a wide range of cells, and is involved in the control of gene expression in cAMP-activated cells. DEC2, also known as SHARP1, is highly expressed in skeletal muscle and brain. The gene encoding human DEC2 maps to chromosome 12p11.23-p12.1. DEC1 and DEC2 play a role in regulating the mammalian molecular clock by suppressing the transcription of specific clock genes. Both DEC1 and DEC2 are detected in the suprachiasmimc nucleus in a circadian fashion. Brief light impulses induce the expression of DEC1 in a phase-dependent manner.Synonyms: Class B basic helix-loop-helix protein 3, Class E basic helix-loop-helix protein 41, DEC2, Differentially expressed in chondrocytes protein 2, Enhancer-of-split and hairy-related protein 1, SHARP-1, SHARP1

Molecular Weight: approx. 50 kDa

Gene ID: 79365

NCBI Accession: NP_110389

UniProt: Q9C0J9

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development