MED17 antibody

Catalog No. ABIN498360

The Rabbit Polyclonal anti-MED17 antibody (ABIN498360) specifically detects MED17 in WB and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for MED17 antibody (ABIN498360)

Target: MED17 (Mediator Complex Subunit 17 (MED17))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED17 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MED17 Antibody

Specificity: This antibody detects endogenous levels of CRSP77 protein. (region surrounding Val171)

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MED17

Target: MED17 (Mediator Complex Subunit 17 (MED17))

Alternative Name: MED17

Background: In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase. These complexes include CRSP (for cofactor required for Sp1 activation), which is required, in conjunction with TAFIIs, for transcriptional activation by Sp1. CRSP is ubiquitously expressed in various tissues and functions as a multimeric complex that consists of nine distinct subunits, each having an individual molecular mass ranging from 33 to 200 kDa. Several members of the CRSP family share sequence similarity with multiple components of the yeast transcriptional mediator proteins, including CRSP150, which is related to yeast Rgr1, and CRSP70, which is similar to the elongation factor TFIIS. CRSP77 and CRSP150 are also related to proteins within the putative murine mediator complex, while CRSP130 and CRSP34 are largely unrelated to either murine or yeast proteins. CRSP subunits also associate with larger multimeric co-activator complexes, including ARC/DRI, which binds directly to SREBP and nuclear hormone receptors to facilitate transcription, and with NAT, a polymerase II-interacting complex.Synonyms: ARC77, Activator-recruited cofactor 77 kDa component, CRSP complex subunit 6, CRSP6, Cofactor required for Sp1 transcriptional activation subunit 6, DRIP77, DRIP80, Mediator complex subunit 17, Mediator of RNA polymerase II transcription subunit 17, TRAP80, Thyroid hormone receptor-associated protein complex 80 kDa component, Transcriptional coactivator CRSP77, Vitamin D3 receptor-interacting protein complex 80 kDa component

Molecular Weight: approx. 73 kDa

Gene ID: 9440

NCBI Accession: NP_004259

UniProt: Q9NVC6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha