ZIC1 antibody

Catalog No. ABIN498412

Product Details anti-ZIC1 Antibody

Target: ZIC1 (Zinc Finger Protein ZIC 1 (ZIC1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZIC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: This antibody detects endogenous levels of Zic1/2/3 protein. (region surrounding Asp348)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.
Also reacts to: ZIC2/3

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ZIC1

Target: ZIC1 (Zinc Finger Protein ZIC 1 (ZIC1))

Alternative Name: ZIC1 (ZIC1 Products)

Synonyms: ZIC antibody, ZNF201 antibody, opl antibody, Zic family member 1 antibody, Zic family member 1 S homeolog antibody, zinc finger protein of the cerebellum 1 antibody, ZIC1 antibody, Zic1 antibody, zic1.S antibody

Background: Zic1 encodes a zinc finger protein expressed in the developing or matured central nervous system in a highly restricted manner. Zic is expressed in granule cells that make synaptic contact with Purkinje cells. Zic1 is a gene critical to cerebellar pattern formation. The expression of Zic genes is first detected at gastrulation and at neurulation, becoming restricted to the dorsal neural ectoderm and the dorsal paraxial mesoderm. The Zic1 gene has been mapped to chromosome 9 in mouse. The 5' flanking region of the Zic1 gene contains a region-specific enhancer determined to be essential in in-vivo and in-vitro deletion analysis. The temporal profile of mRNA expression differs for each of the Zic gene products. The Drosophila odd-paired gene is highly homologous to the Zic gene family. Zic2 and Zic3 are highly similar genes. Zic2 is essential for the formation of the brain and Zic-3 is important for right and left axis formation.Synonyms: ZIC, Zinc finger protein ZIC 1, Zinc finger protein of the cerebellum 1

Molecular Weight: approx. 51 kDa

Gene ID: 7545

NCBI Accession: NP_003403

UniProt: Q15915