DGKQ antibody

Catalog No. ABIN498426

The Rabbit Polyclonal anti-DGKQ antibody (ABIN498426) specifically detects DGKQ in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for DGKQ antibody (ABIN498426)

Target: DGKQ (Diacylglycerol Kinase, theta 110kDa (DGKQ))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGKQ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DGKQ Antibody

Specificity: This antibody reacts to DGKQ.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DGKQ

Target: DGKQ (Diacylglycerol Kinase, theta 110kDa (DGKQ))

Alternative Name: DAG Kinase theta

Background: Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. The accumulation of DAG in the nucleus is important for the regulation of cell growth and differentiation. Diacylglycerol kinases (DGKs) convert DAG to phosphatidic acid, thereby terminating diacylglycerol signaling, which results in the reduction of protein kinase C activity and cell cycle progression of T lymphocytes. Diacylglycerol kinases are divided into five subtypes, Type I-Type V. DGK-theta is a Type V DGK, and localizes mainly to the nucleus of various cell lines, such as MDA-MB-453, MCF-7, PC12 and HeLa. Nuclear DGK-theta co-localizes with phosphatidylinositol 4,5-bisphosphate (PIP(2)). DGK-theta is the isoform responsive to alpha-Thrombin stimulation.Synonyms: DAGK4, DGK-theta, DGKQ, Diacylglycerol kinase theta, Diglyceride kinase theta

Molecular Weight: approx. 101 kDa

Gene ID: 1609

NCBI Accession: NP_001338

UniProt: P52824