GAL4 antibody

Catalog No. ABIN498445

This Rabbit Polyclonal antibody specifically detects GAL4 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for GAL4 antibody (ABIN498445)

Target: GAL4 (LGALS4) (Galectin 4 (LGALS4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAL4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GAL4 Antibody

Specificity: This antibody detects endogenous levels of Galectin-4 protein. (region surrounding Lys83)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GAL4

Target: GAL4 (LGALS4) (Galectin 4 (LGALS4))

Alternative Name: Galectin-4

Background: Galectins are a family of soluble beta-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One member of this family, Galectin-4, also known as Gal-4, L36 or LGALS4 maps to human chromosome 19q13.13 and encodes a 36-37 kDa protein. The Galectin-4 protein is composed of 323 amino acids and contains two homologous carbohydrate recognition domains (CRD) and all amino acids typically conserved in the galectin family. Expression of Galectin-4 correlates with the malignant potential of human hepatocellular carcinoma (HCC) and is differentially regulated depending on cell-cell contact, serum growth factors, cell growth and cell differentiation status. Galectin-4 expression is detected in epithelial cells of the colon, rectum, intestine, and in HT29 and LS174T cell lines. Galectin-4 is underexpressed in colorectal cancer and is preferentially upregulated in cells prone to peritoneal dissemination.Synonyms: Antigen NY-CO-27, Galectin4, L-36 lactose-binding protein, L36LBP, LGALS4, Lactose-binding lectin 4

Molecular Weight: approx. 36 kDa

Gene ID: 3960

NCBI Accession: NP_006140

UniProt: P56470