Granzyme H (Cathepsin G-Like 2, Protein H-CCPX) (GZMH) antibody

Details for Product No. ABIN498458
Target
Reactivity
Human
32
11
3
1
Host
Rabbit
27
5
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
23
14
11
9
6
4
2
1
1
1
Options
Specificity This antibody detects endogenous levels of Granzyme H protein. (region surrounding Gly75)
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE
Target
Alternative Name Granzyme H (GZMH) (GZMH Antibody Abstract)
Background The granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and granzyme B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis and, thereby, mediate apoptosis through two different pathways. Granzyme H, also designated cytotoxic T-lymphocyte proteinase, cathepsin G-like 2 (CTSGL2) or cytotoxic serine protease C (CSP-C), contains one peptidase S1 domain. Granzyme H localizes to cytoplasmic granules of cytolytic T-lymphocytes and is important for target cell lysis in cell-mediated immune responsesSynonyms: CCP-X, CGL2, CSP-C, CTSGL2, Cathepsin G-like 2, Cytotoxic T-lymphocyte proteinase, Cytotoxic serine protease C
Molecular Weight approx. 23 kDa
Gene ID 2999
NCBI Accession NP_219491
UniProt P20718
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Images
Image no. 1 for anti-Granzyme H (Cathepsin G-Like 2, Protein H-CCPX) (GZMH) antibody (ABIN498458) Western blot (WB) analysis of Granzyme H antibody in extracts from K562 cells.
Image no. 2 for anti-Granzyme H (Cathepsin G-Like 2, Protein H-CCPX) (GZMH) antibody (ABIN498458) Western blot (WB) analysis of Granzyme H antibody  at 1/500 dilution. Lane 1: MCF-7 w...
Image no. 3 for anti-Granzyme H (Cathepsin G-Like 2, Protein H-CCPX) (GZMH) antibody (ABIN498458) Immunohistochemistry (IHC) analyzes of Granzyme H antibody in paraffin-embedded human...