Cortactin antibody

Catalog No. ABIN498636

The Rabbit Polyclonal anti-Cortactin antibody (ABIN498636) specifically detects Cortactin in WB, IF and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for Cortactin antibody (ABIN498636)

Target: Cortactin (CTTN)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cortactin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cortactin Antibody

Specificity: This antibody detects endogenous levels of Cortactin protein. (region surrounding Tyr460)

Cross-Reactivity (Details): Species reactivity (tested):Mouse, Rat.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Cortactin

Target: Cortactin (CTTN)

Alternative Name: Cortactin

Background: Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.Synonyms: Amplaxin, CTTN, EMS1, Oncogene EMS1

Molecular Weight: approx. 85.0 kDa

Gene ID: 2017

NCBI Accession: NP_005222

UniProt: Q14247

Pathways: MAPK Signaling