ASCL1 antibody

Catalog No. ABIN498679

This Rabbit Polyclonal antibody specifically detects ASCL1 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ASCL1 antibody (ABIN498679)

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASCL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ASCL1 Antibody

Specificity: This antibody detects endogenous levels of ASCL1 protein. (region surrounding Glu158)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ASCL1

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Alternative Name: ASCL1

Background: The mammalian homolog of the Drosophila protein achaetescute, ASCL1 (also known as ASH1) is a basic helix-loophelix transcription factor that is required for early development of the nervous system. Expressed in fetal brain, ASCL1 is essential for the proper development of autonomic neurons and for the survival of subsets of autonomic neurons. ASCL1 interaction with MEF-2A may regulate the expression of specific genes that are critical for the formation of distinct neuronal circuits within the central nervous system. The high level of ASCL1 expression in neuroendocrine tumors, such as medullary thyroid cancer, small cell lung cancer and lung cancer with neuroendocrine features may provide a useful marker for cancers with neuroendocrine features. Mapping to human chromosome 12, the ASCL1 gene contains a trinucleotide repeat region, making this locus a candidate for inherited disease.Synonyms: ASH1, Achaete-scute homolog 1, HASH1, MASH1

Molecular Weight: approx. 25 kDa

Gene ID: 429

NCBI Accession: NP_004307

UniProt: P50553

Pathways: Dopaminergic Neurogenesis