Cortactin antibody (pTyr421)

Catalog No. ABIN498755

The Rabbit Polyclonal anti-Cortactin antibody is suitable to detect Cortactin in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for Cortactin antibody (pTyr421) (ABIN498755)

Target: Cortactin (CTTN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cortactin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cortactin Antibody

Binding Specificity: pTyr421

Specificity: This antibody detects endogenous levels of Cortactin protein only when phosphorylated at Tyr421.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Cortactin

Target: Cortactin (CTTN)

Alternative Name: Cortactin

Background: Cortactin (also designated Ems-1) is a filamentous Actin (FActin) binding protein. It has also been shown to be a substrate for Src p60. Cortactin contains tandem 37 amino acid repeats at the amino-terminus and an SH3 domain at the carboxy-terminus. The tandem repeats appear to be necessary for F-Actin binding. Tyrosine phosphorylation of Cortactin by Src p60 results in diminished F-Actin binding to Cortactin and reduced F-Actin cross-linking activity. Cortactin has also been shown to be phosphorylated in response to FGF-1. Cortactin exhibits abundant expression in megakaryocytes and platelets, and it may play a role in the maturation of megakaryocytes.Synonyms: Amplaxin, CTTN, EMS1, Oncogene EMS1

Molecular Weight: approx. 85.0 kDa

Gene ID: 2017

NCBI Accession: NP_005222

UniProt: Q14247

Pathways: MAPK Signaling