PKC mu antibody

Catalog No. ABIN498890

The Rabbit Polyclonal anti-PKC mu antibody is suitable to detect PKC mu in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for PKC mu antibody (ABIN498890)

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC mu antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PKC mu Antibody

Specificity: This antibody detects endogenous levels of PKD1/PKC-mu protein. (region surrounding Val744)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PKC mu

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Alternative Name: PRKD1

Background: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, epsilon, zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilon are independent of Ca++. On the other hand, nPKC delta and epsilon, as well as all of the cPKC members, possess phorbol esterbinding activities and kinase activities.Synonyms: PKC D1, PKC mu, PKD, PKD1, PRKCM, Protein kinase C mu type, Protein kinase D, Serine/threonine-protein kinase D1, nPKC-D1, nPKC-mu

Molecular Weight: approx. 100 to 140 kDa

Gene ID: 5587

NCBI Accession: NP_002733

UniProt: Q15139

Pathways: Myometrial Relaxation and Contraction