NPM1 antibody

Catalog No. ABIN498955

This Rabbit Polyclonal antibody specifically detects NPM1 in WB, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NPM1 antibody (ABIN498955)

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NPM1 Antibody

Specificity: This antibody detects endogenous levels of NPM protein. (region surrounding Lys230)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name: Nucleophosmin

Background: This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.Synonyms: NPM, NPM1, Nucleolar Phosphoprotein B23, Nucleolar Protein NO38, Numatrin

Molecular Weight: approx. 33 kDa

Gene ID: 4869

NCBI Accession: NP_002511

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly