FYN antibody

Catalog No. ABIN498956

The Rabbit Polyclonal anti-FYN antibody is suitable to detect FYN in samples from Human and Mouse. It has been validated for WB.

Quick Overview for FYN antibody (ABIN498956)

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FYN antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FYN Antibody

Specificity: This antibody detects endogenous levels of Fyn protein. (region surrounding Thr524)

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FYN

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Alternative Name: FYN

Background: Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59 kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.Synonyms: Proto-oncogene tyrosine-protein kinase Fyn, Protooncogene Syn, SLK, p59-Fyn

Molecular Weight: approx. 59 kDa

Gene ID: 2534

NCBI Accession: NP_002028

UniProt: P06241

Pathways: JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling