POLD1 antibody

Catalog No. ABIN499061

The Rabbit Polyclonal anti-POLD1 antibody is suitable to detect POLD1 in samples from Human, Rat and Mouse. It has been validated for WB.

Quick Overview for POLD1 antibody (ABIN499061)

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLD1 Antibody

Specificity: This antibody detects endogenous levels of CNA pol delta cat protein. (region surrounding Asp1091)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity-Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western blot: 1: 500-1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for POLD1

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Alternative Name: POLD1

Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and togther with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity).Synonyms: DNA polymerase delta catalytic subunit, DNA polymerase subunit delta p125

Molecular Weight: approx. 125 kDa

Gene ID: 5424

NCBI Accession: NP_002682

UniProt: P28340

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Chromatin Binding, Synthesis of DNA