MYO5A antibody (Internal Region)

Catalog No. ABIN499147

This Goat Polyclonal antibody specifically detects MYO5A in WB, IF and EIA. It exhibits reactivity toward Human, Mouse, Rat and Cow.

Quick Overview for MYO5A antibody (Internal Region) (ABIN499147)

Target: MYO5A (Myosin VA (MYO5A))

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This MYO5A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-MYO5A Antibody

Binding Specificity: Internal Region

Sequence: C-ETKQLELDLN

Specificity: This antibody is expected to recognize both reported isoforms (NP_000250.3 and NP_001135967.1).

Cross-Reactivity (Details): Species reactivity (expected):Rat, Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide sequence from the internal region of the protein sequence according to NP_000250.3, NP_001135967.1. Genename: MYO5A

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 1-3 μg/mL. This antibody detects a band of approx. 200 kDa in lysates of cell lineJurkat.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MYO5A

Target: MYO5A (Myosin VA (MYO5A))

Alternative Name: Myosin-Va

Background: Myosin-Va has been implicated in the regulation of vesicle trafficking in neurons and melanocytes. It has been suggested that class V myosins associate with membrane vesicles through their C-terminal tails. Myosin V associates with synaptic vesicles and forms a stable complex with the synaptic vesicle membrane proteins, synaptobrevin II, and synaptophysin. The dilute Myo5a gene mutation in myosin Va leads to mental retardation, variable cellular immunodeficiency and impaired melanocyte function (pigmentary dilution), known as Griscelli syndrome in humans. Myosin Va regulates melanosome distribution along microfilaments. It is found in association with the centrosome at all stages of the cell cycle. In the interphase stage, myosin Va is found in pericentriolar material. During cell division, it is found in the cytoplasm and concentrates in a trail between migrating centrioles and in the mitotic spindle poles and spindle fibers.Synonyms: Dilute myosin heavy chain, MYH12, MYO5A, Myosin heavy chain 12, Myoxin, non-muscle

Molecular Weight: 215kDa (NP_000250.3).

Gene ID: 4644

NCBI Accession: NP_000250

UniProt: Q9Y4I1

Pathways: Hormone Transport, Peptide Hormone Metabolism