CDC27 antibody (C-Term)

Catalog No. ABIN499295

Product Details anti-CDC27 Antibody

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC27 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Purification: Affinity chromatography purified via peptide column

Immunogen: APC3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human APC3.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: APC3 antibody can be used for detection of APC3 at 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for CDC27

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Alternative Name: CDC27 (CDC27 Products)

Synonyms: ANAPC3 antibody, APC3 antibody, CDC27Hs antibody, D0S1430E antibody, D17S978E antibody, HNUC antibody, NUC2 antibody, wu:fb54g06 antibody, wu:fc88c12 antibody, AI452358 antibody, BC023187 antibody, CDC27 antibody, NV10210 antibody, cell division cycle 27 antibody, cell division cycle 27 S homeolog antibody, cell division cycle protein 27 homolog antibody, anaphase-promoting complex subunit Apc3 antibody, CDC27 antibody, cdc27 antibody, Cdc27 antibody, cdc27.S antibody, LOC100123129 antibody, nuc2 antibody

Background: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC3, also known as CDC27, a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), is a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. APC3 contains a tetratricopeptide repeat (TPR) region and interacts with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1.Synonyms: CDC27Hs, Cell division cycle protein 27 homolog, D0S1430E, D17S978E, H-NUC

Gene ID: 996

NCBI Accession: NP_001247

UniProt: P30260