PYCARD antibody

Catalog No. ABIN499343

The Rabbit Polyclonal anti-PYCARD antibody has been validated for WB, IF and EIA. It is suitable to detect PYCARD in samples from Human.

Quick Overview for PYCARD antibody (ABIN499343)

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYCARD antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-PYCARD Antibody

Specificity: ASC antibody was raised against a synthetic peptide corresponding to amino acids 182 to 195 of human ASC .

Purification: Affinity chromatography purified via peptide column

Immunogen: Human ASC Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: ASC antibody can be used for detection of ASC/TMS1 at 1 μg/mL. Anapproximately 25 kDa band can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: CARD5 / PYCARD / ASC

Background: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1 (1-3). Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3,4). Overexpression of ASC/TMS1 induced DNA fragmentation (4). ASC/TMS1 is expressed in a variety of human and mouse tissues (1, 2)Synonyms: Caspase recruitment domain-containing protein 5, PYD and CARD domain-containing protein, TMS1, Target of methylation-induced silencing 1, poptosis-associated speck-like protein containing a CARD

Gene ID: 29108

UniProt: Q9ULZ3

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome