BID antibody (Middle Region)

Catalog No. ABIN499463

The Rabbit Polyclonal anti-BID antibody has been validated for WB and EIA. It is suitable to detect BID in samples from Human and Mouse.

Quick Overview for BID antibody (Middle Region) (ABIN499463)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BID antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-BID Antibody

Binding Specificity: Middle Region

Specificity: Bid antibody was raised against a peptide corresponding to 14 amino acids near the middle of human Bid.

Purification: Ion exchange chromatography

Immunogen: Human Bid (IN) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Bid antibody can be used for detection of Bid at 1 to 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID

Background: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death (reviewed in 2 and 3). Bid, a pro-apoptotic Bcl-2 family member, is cleaved by caspase-8 in response to apoptotic signals (4,5), exposing the Bcl-2 homology 3 (BH3) domain which is normally buried in the full-length protein (6). The cleaved complex is myris-toylated and translocated to the mitochondrial membrane where it may induce mitochondrial Bax and Bak to oligomerize (7,8).Synonyms: BH3-interacting domain death agonist, p22 BID

Gene ID: 637

UniProt: P55957

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity