Caspase 1 antibody (Intermediate Domain)

Catalog No. ABIN499543

This Rabbit Polyclonal antibody specifically detects Caspase 1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for Caspase 1 antibody (Intermediate Domain) (ABIN499543)

Target: Caspase 1 (CASP1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Caspase 1 Antibody

Binding Specificity: Intermediate Domain

Specificity: Caspase-1 antibody was raised against a 15 amino acid peptide from near the middle of human Caspase-1.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human Casp-1 / ICE / IL-1b converting enzyme (Intermediate Domain) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Casp-1 antibody can be used for the detection of Caspase-1 at 0.5 μg/mL. Depending on cell lines or tissues used, other cleavage products may be observed. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: Caspase-1

Background: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1 and 2). Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1beta and IL-18 (3,4). Caspase-1 was initially identified as an IL-1beta-converting enzyme (5), later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 (6) whose overexpression can induce apoptosis in fibroblasts (7).Synonyms: CASP-1, CASP1, ICE, IL1BC, IL1BCE, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45

Gene ID: 834

NCBI Accession: NP_150634

UniProt: P29466

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome