AACS antibody (AA 182-228) (AbBy Fluor® 680)

Catalog No. ABIN4995823

The Rabbit Polyclonal anti-AACS antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect AACS in samples from Human.

Quick Overview for AACS antibody (AA 182-228) (AbBy Fluor® 680) (ABIN4995823)

Target: AACS (Acetoacetyl-CoA Synthetase (AACS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AACS antibody is conjugated to AbBy Fluor® 680

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-AACS Antibody (AbBy Fluor® 680)

Binding Specificity: AA 182-228

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human AACS

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for AACS

Target: AACS (Acetoacetyl-CoA Synthetase (AACS))

Alternative Name: AACS/ACSF1

Background:

Synonyms: Acetoacetyl-CoA synthetase, ACSF1, Acyl-CoA synthetase family member 1, Protein sur-5 homolog, AACS_HUMAN.

Background: ACSF1 is a 672 amino acid protein belonging to the ATP-dependent AMP-binding enzyme family. Encoded by a gene that maps to human chromosome 12q24.31, ACSF1 is highly expressed in kidney, heart and brain, and shows similar neural expression as HMGCR (3-hydroxy-3-methylglutaryl-CoA reductase). Existing as three alternatively spliced isoforms, ACSF1 participates in ATP binding, ligase activity, acetoacetate-CoA ligase activity and nucleotide binding. The ACSF1 promoter is a known PPAR?target gene, with the nuclear receptor recruited to the ACSF1 promoter by direct interaction with stimulating protein-1 (Sp1). ACSF1 activates acetoacetate and is highly regulated by modulators that affect HMGCR and cholesterol biosynthesis.

Gene ID: 65985

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis