ATF2 antibody (pSer322) (AbBy Fluor® 750)

Catalog No. ABIN4996960

The Rabbit Polyclonal anti-ATF2 antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect ATF2 in samples from Mouse and Rat.

Quick Overview for ATF2 antibody (pSer322) (AbBy Fluor® 750) (ABIN4996960)

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF2 antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ATF2 Antibody (AbBy Fluor® 750)

Binding Specificity: pSer322

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse ATF2 around the phosphorylation site of Ser322

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ATF2

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Alternative Name: ATF2

Background:

Synonyms: ATF2 phospho Ser322, ATF2 phospho S322, p-ATF2 S322, p-ATF2 Ser322, CREB 2, HB 16,Activating Transcription Factor 2, ATF 2, Atf-2, ATF2 protein, cAMP Response Element Binding Protein 2, cAMP response element binding protein CRE BP1, cAMP-dependent transcription factor ATF-2, cAMP-responsive element-binding protein 2, CRE BP1, CRE-BP, CREB 2, CREB2, CREBP1, Cyclic AMP dependent transcription factor ATF 2, Cyclic AMP-responsive, ATF2_MOUSE.

Background: ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

Gene ID: 11909

Pathways: MAPK Signaling, RTK Signaling, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades