ATG3 antibody (pTyr18) (AbBy Fluor® 680)

Catalog No. ABIN4996988

This anti-ATG3 antibody is a Rabbit Polyclonal antibody detecting ATG3 in WB, IF (cc) and IF (p). Suitable for Human.

Quick Overview for ATG3 antibody (pTyr18) (AbBy Fluor® 680) (ABIN4996988)

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG3 antibody is conjugated to AbBy Fluor® 680

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ATG3 Antibody (AbBy Fluor® 680)

Binding Specificity: pTyr18

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ATG3 around the phosphorylation site of Tyr18

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ATG3

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Alternative Name: ATG3

Background:

Synonyms: 2610016C12Rik, Apg 3, APG3, APG3 autophagy 3 like, APG3 like, APG3-like, APG3L, Apg3p, ATG 3, ATG3, ATG3 autophagy related 3 homolog, ATG3 autophagy related 3 homolog S. cerevisiae, ATG3_HUMAN, Autophagy Apg3p/Aut1p like, Autophagy related protein 3, Autophagy-related protein 3, DKFZp564M1178, FLJ22125, hApg3, MGC15201, OTTHUMP00000214547, OTTHUMP00000214548, PC3 96, Protein PC3-96, Ubiquitin-like-conjugating enzyme ATG3.

Background: E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.

Gene ID: 64422

Pathways: Autophagy