DFFA antibody (C-Term)

Catalog No. ABIN499742

The Rabbit Polyclonal anti-DFFA antibody is suitable to detect DFFA in samples from Human. It has been validated for WB, IF and EIA.

Quick Overview for DFFA antibody (C-Term) (ABIN499742)

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DFFA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-DFFA Antibody

Binding Specificity: C-Term

Specificity: DFF45 antibody was raised against a peptide corresponding to amino acids 313 to 331 of human DFF45.

Purification: DEAE

Immunogen: Human DFF45 (C-Terminus) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: DFF45 antibody can be used for detection of DFF45 and one of the cleavedfragments of DFF45 at 1/1000 to 1/2000 dilution. A 45 kDa band can be detected innon-apoptotic cells. Immunocytochemistry. Immunoprecipitation: DFF45 antibody can be used for detection of DFF45 and one of thecleaved fragments of DFF45 at 1/1000 to 1/2000 dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for DFFA

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Alternative Name: DFFA / ICAD

Background: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD (2,3). Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD

Gene ID: 1676

NCBI Accession: NP_004392

UniProt: O00273

Pathways: Apoptosis, Caspase Cascade in Apoptosis