BID antibody (pSer61) (AbBy Fluor® 680)

Catalog No. ABIN4997454

This Rabbit Polyclonal antibody specifically detects BID in WB, FACS, IF (cc) and IF (p). It exhibits reactivity toward Human.

Quick Overview for BID antibody (pSer61) (AbBy Fluor® 680) (ABIN4997454)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BID antibody is conjugated to AbBy Fluor® 680

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-BID Antibody (AbBy Fluor® 680)

Binding Specificity: pSer61

Cross-Reactivity: Human

Predicted Reactivity: Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse Bid around the phosphorylation site of Ser61

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: Bid

Background:

Synonyms: Bid phospho S61, p-Bid phospho S61, BH3 interacting domain death agonist, p22 BID, BID, BH3-interacting domain death agonist, AltName: BH3-interacting domain death agonist p15, BH3-interacting domain death agonist p13, p13 BID, BH3-interacting domain death agonist p11, p11 BID.

Background: Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

Gene ID: 12122

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity