ECSIT antibody (C-Term)

Catalog No. ABIN499780

This Rabbit Polyclonal antibody specifically detects ECSIT in WB, EIA and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for ECSIT antibody (C-Term) (ABIN499780)

Target: ECSIT

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECSIT antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ECSIT Antibody

Binding Specificity: C-Term

Specificity: ECSIT antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human ECSIT.

Purification: Ion exchange chromatography

Immunogen: Human ECSIT (C-Terminus) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 to 1 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for ECSIT

Target: ECSIT

Alternative Name: ECSIT

Background: Activation of NF-?B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.Synonyms: ECSIT homolog (Drosophila), Evolutionarily conserved signaling intermediate in Toll pathway, SITPEC, Signaling intermediate in toll pathway evolutionarily conserved, mitochondrial

Gene ID: 51295

NCBI Accession: NP_057665

UniProt: Q9BQ95

Pathways: TLR Signaling, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome