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DFFA antibody (N-Term)

DFFA Reactivity: Mouse WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN499956
  • Target See all DFFA Antibodies
    DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
    Binding Specificity
    • 15
    • 14
    • 9
    • 8
    • 6
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 74
    • 32
    • 20
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 75
    • 9
    • 1
    Rabbit
    Clonality
    • 77
    • 8
    Polyclonal
    Conjugate
    • 61
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This DFFA antibody is un-conjugated
    Application
    • 82
    • 30
    • 15
    • 14
    • 13
    • 12
    • 12
    • 10
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    ICAD antibody was raised against a peptide corresponding to amino acids near the amino terminus of mouse ICAD .
    Purification
    Affinity chromatography purified via peptide column
    Immunogen
    Mouse ICAD (N-Terminus) Peptide
    Isotype
    IgG
    Top Product
    Discover our top product DFFA Primary Antibody
  • Application Notes
    ELISA. Western Blot: 0.5 - 1 μg/mL. A 45 kDa band can be detected. Immunohistochemistry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Buffer
    PBS containing 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C.
  • Target
    DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
    Alternative Name
    DFFA / ICAD (DFFA Products)
    Synonyms
    DFF-45 antibody, DFF1 antibody, ICAD antibody, A330085O09Rik antibody, DFF35 antibody, Dff45 antibody, ICAD-L antibody, ICAD-S antibody, DFFA antibody, zgc:92229 antibody, DNA fragmentation factor subunit alpha antibody, DNA fragmentation factor, alpha subunit antibody, DNA fragmentation factor, 45kDa, alpha polypeptide antibody, DNA fragmentation factor, alpha polypeptide antibody, DFFA antibody, Dffa antibody, dffa antibody
    Background
    Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which is cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD
    Gene ID
    13347
    UniProt
    O54786
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis
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