IFIH1 antibody (C-Term)

Catalog No. ABIN500264

The Rabbit Polyclonal anti-IFIH1 antibody is suitable to detect IFIH1 in samples from Human and Mouse. It has been validated for WB, IF, IHC (p) and EIA.

Quick Overview for IFIH1 antibody (C-Term) (ABIN500264)

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFIH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-IFIH1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects Helicard at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Peptide Affinity Chromatography

Immunogen: A 16 amino acid peptide from near the carboxy terminus of Human MDA5.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IFIH1

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Alternative Name: Helicard

Background: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.Synonyms: Helicase with 2 CARD domains, IFIH1, Interferon-induced helicase C domain-containing protein 1, MDA5, Melanoma differentiation-associated protein 5, Murabutide down-regulated protein, RH116, RNA helicase-DEAD box protein 116

Gene ID: 64135

NCBI Accession: NP_071451

UniProt: Q9BYX4

Pathways: Activation of Innate immune Response