TBK1 antibody (C-Term)

Catalog No. ABIN500308

This Rabbit Polyclonal antibody specifically detects TBK1 in WB, IF and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for TBK1 antibody (C-Term) (ABIN500308)

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-TBK1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects TBK1 at C-term. It has no cross response to IKKa, IKKb, IKKy, or IKKe.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Peptide affinity chromatography

Immunogen: Peptide from C-term of human NAK / TBK1 (Cat. No. 2351P)

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL, an 84 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for TBK1

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Alternative Name: TBK1

Background: Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappaB is associated with IkappaB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. Phosphorylation of I-kappaB by IkappaB kinase (IKK) complex leads to degradation of I-kappaB and activation of NF-kappaB. The IKK complex contains IKKalpha, IKKbeta, and IKKgamma. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-kappaB-activating kinase), and T2K (1-3). NAK/TBK1 activates IKKbeta through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kappaB activation in response to growth factors (2). NAK/TBK1 functions upstream of NIK and the IKK complex (1,2). NAK/TBK1 is also critical in protecting embryonic liver from apoptosis (3).Synonyms: NAK, NF-kappa-B-activating kinase, T2K, TANK-binding kinase 1

Gene ID: 29110

NCBI Accession: NP_037386

UniProt: Q9UHD2

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome