HA-Tag antibody

Catalog No. ABIN5004206

The Mouse Monoclonal anti-HA-Tag antibody (Clone 7H5) (ABIN5004206) specifically detects HA-Tag in WB, IP and IF (cc). The antibody is reactive with Human and Various Species samples.

Quick Overview for HA-Tag antibody (ABIN5004206)

Target: HA-Tag

Reactivity: Human, Various Species

Host: Mouse

Clonality: Monoclonal

Conjugate: This HA-Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 7H5

Product Details anti-HA-Tag Antibody

Cross-Reactivity: Human, Various Species

Purification: Ammonium sulfate precipitation

Immunogen: Recombinant Human HA-tag protein purified E.coli

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IF(ICC) 1:50-200
IP(1-2 μg)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for HA-Tag

Target: HA-Tag

Alternative Name: HA tag

Target Type: Tag

Background: Optional[synonyms]: Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage. The expression vectors are used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. Short pieces of welldefined peptides(Poly-His, Flag-epitope or cmyc epitope or HA-tag) or small proteins (bacterial GST, MBP) is often cloned along with the target gene. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors. It has been successfully used in a variety of applications, including Western blotting, Immunoprecipitation, and immunofluorescence studies.