PD-1 antibody (Center)

Catalog No. ABIN500459

The Rabbit Polyclonal anti-PD-1 antibody is suitable to detect PD-1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC (p) and EIA.

Quick Overview for PD-1 antibody (Center) (ABIN500459)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PD-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PD-1 Antibody

Binding Specificity: Center

Specificity: This antibody detects CD279 / PD1 at center.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: PD-1 antibody was raised against a 16 amino acid peptide from near the center of human PD-1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: CD279 / PD1

Background: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of Synonyms: PDCD1, Programmed cell death protein 1, Protein PD-1, hPD-1

Gene ID: 5133

UniProt: Q15116

Pathways: Cancer Immune Checkpoints