His Tag antibody (Biotin)

Catalog No. ABIN5004652

The Mouse Monoclonal anti-His Tag antibody has been validated for WB. It is suitable to detect His Tag in samples from Human.

Quick Overview for His Tag antibody (Biotin) (ABIN5004652)

Target: His Tag

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to Biotin

Application: Western Blotting (WB)

Clone: 6E6

Product Details anti-His Tag Antibody (Biotin)

Cross-Reactivity: Human

Purification: Purified by Protein G.

Immunogen: Human, fusion protein HHHHHH tagged

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Target Type: Tag

Background:

Synonyms: HisX6, 6His, His Tag, 6 His epitope tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Histidine.

Background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.