PIAS3 antibody (C-Term)

Catalog No. ABIN500491

This Rabbit Polyclonal antibody specifically detects PIAS3 in WB and EIA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for PIAS3 antibody (C-Term) (ABIN500491)

Target: PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PIAS3 Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: PIAS3 antibody was raised against a 12 amino acid peptide near the carboxy terminus of human PIAS3.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for PIAS3

Target: PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))

Alternative Name: PIAS3

Background: The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS3. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS3 binds specifically to STAT3 following the stimulation of STAT3. Increased expression of PIAS3 has been observed in several human cancers, including lung, breast, and brain tumors, but not in anaplastic lymphoma kinase-positive T/null-cell lymphomas, indicating that PIAS3 plays multiple roles in different tissue and cell types.Synonyms: E3 SUMO-protein ligase PIAS3, Protein inhibitor of activated STAT protein 3

Gene ID: 10401

NCBI Accession: NP_006090

UniProt: Q9Y6X2

Pathways: JAK-STAT Signaling