PIGY antibody (C-Term)

Catalog No. ABIN500497

This Rabbit Polyclonal antibody specifically detects PIGY in WB, IHC (p) and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for PIGY antibody (C-Term) (ABIN500497)

Target: PIGY (Phosphatidylinositol Glycan Anchor Biosynthesis, Class Y (PIGY))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGY antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PIGY Antibody

Binding Specificity: C-Term

Specificity: This antibody detects PIG-Y at C-term. It only detects isoform 1 of PIG-Y.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Peptide affinity chromatography

Immunogen: PIG-Y antibody was raised against a 16 amino acid peptide near the carboxy terminus of human PIG-Y.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for PIGY

Target: PIGY (Phosphatidylinositol Glycan Anchor Biosynthesis, Class Y (PIGY))

Alternative Name: PIGY

Background: Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE.Synonyms: PIG-Y, Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y, Phosphatidylinositol-glycan biosynthesis class Y protein

Gene ID: 84992

NCBI Accession: NP_001036081

UniProt: Q3MUY2

Pathways: Inositol Metabolic Process