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PIGY antibody (AA 50-100)

PIGY Reactivity: Human, Mouse ELISA, WB, IHC (p), IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6991033
  • Target See all PIGY Antibodies
    PIGY (Phosphatidylinositol Glycan Anchor Biosynthesis, Class Y (PIGY))
    Binding Specificity
    • 2
    • 2
    • 1
    AA 50-100
    Reactivity
    Human, Mouse
    Host
    • 5
    Rabbit
    Clonality
    • 5
    Polyclonal
    Conjugate
    • 5
    This PIGY antibody is un-conjugated
    Application
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Specificity
    This antibody only detects isoform 1 of PIG-Y.
    Purification
    PIG-Y Antibody is affinity chromatography purified via peptide column.
    Immunogen
    PIG-Y antibody was raised against a 16 amino acid synthetic peptide near the center of human PIG-Y. The immunogen is located within amino acids 50 - 100 of PIG-Y.
    Isotype
    IgG
    Top Product
    Discover our top product PIGY Primary Antibody
  • Application Notes
    PIG-Y antibody can be used for detection of PIG-Y by Western blot at 1 - 2 μ,g/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

    Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PIG-Y Antibody is supplied in PBS containing 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C,4 °C
    Storage Comment
    PIG-Y antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target
    PIGY (Phosphatidylinositol Glycan Anchor Biosynthesis, Class Y (PIGY))
    Alternative Name
    PIG-Y (PIGY Products)
    Synonyms
    PIG-Y antibody, pig-y antibody, Prey antibody, Pyurf antibody, prey antibody, 1810008A14Rik antibody, Pigy antibody, phosphatidylinositol glycan anchor biosynthesis class Y antibody, phosphatidylinositol glycan anchor biosynthesis, class Y antibody, Pigy upstream reading frame S homeolog antibody, phosphatidylinositol glycan anchor biosynthesis, class Y-like antibody, PIGY antibody, pigy antibody, Pigy antibody, pyurf.S antibody, Pigyl antibody
    Background
    PIG-Y Antibody: Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.
    Molecular Weight
    Predicted: 8 kDa

    Observed: 16 kDa
    Gene ID
    84992
    NCBI Accession
    NP_116295
    UniProt
    Q3MUY2
    Pathways
    Inositol Metabolic Process
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