RIPK2 antibody (N-Term)

Catalog No. ABIN500599

This Rabbit Polyclonal antibody specifically detects RIPK2 in WB, IF and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RIPK2 antibody (N-Term) (ABIN500599)

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-RIPK2 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects RICK at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: RICK antibody was raised against a peptide corresponding to amino acids near the amino terminus of human RICK.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1: 1000, an approximately 60 kDa band can be detected. Immuoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for RIPK2

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Alternative Name: RIPK2

Background: Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively, (1-3). RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-kappaB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1b. The messenger RNA of RICK is expressed in multiple human tissues.Synonyms: CARD-containing IL-1 beta ICE-kinase, CARDIAK, RICK, RIP-2, RIP-like-interacting CLARP kinase, RIP2, Receptor-interacting protein 2, Receptor-interacting serine/threonine-protein kinase 2

Gene ID: 8767

UniProt: O43353

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades