RIPK3 antibody (AA 473-486)

Catalog No. ABIN500612

The Rabbit Polyclonal anti-RIPK3 antibody is suitable to detect RIPK3 in samples from Mouse and Rat. It has been validated for WB, IHC (p), IF and EIA.

Quick Overview for RIPK3 antibody (AA 473-486) (ABIN500612)

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-RIPK3 Antibody

Binding Specificity: AA 473-486

Specificity: This antibody detects RIP3.

Cross-Reactivity (Details): Species reactivity (tested):Mouse, Rat.

Purification: Peptide Affinity Chromatography

Immunogen: RIP3 antibody was raised against a peptide corresponding to amino acids 473 to 486 of Murine RIP3.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 to 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RIPK3

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Alternative Name: RIPK3 / RIP3

Background: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3 (1-3). RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-kB activation. Overexpression of RIP3 induces apoptosis and NF-kappaB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues (2,3).Synonyms: RIP-3, RIP-like protein kinase 3, Receptor-interacting protein 3, Receptor-interacting serine/threonine-protein kinase 3

Molecular Weight: 57 kDa

Gene ID: 56532

UniProt: Q9QZL0

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades