DDIT4 antibody (N-Term)

Catalog No. ABIN500644

This Rabbit Polyclonal antibody specifically detects DDIT4 in WB and EIA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for DDIT4 antibody (N-Term) (ABIN500644)

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDIT4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-DDIT4 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects DDIT4 at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: RTP801 antibody was raised against a 14 amino acid peptide from near the amino terminus of human DDIT4.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for DDIT4

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Alternative Name: DDIT4

Background: RTP801 was initially identified as a gene induced by DNA damage, and later found to also be regulated by other cellular stresses such as hypoxia and glucocorticoid treatment. Recently, RTP801 has been shown to act as a mediator of tuberous sclerosis complex (TSC)-dependent regulation of the mammalian Target of Rapamycin (mTOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle. In response to energy stress, RTP801 inhibits mTOR function, resulting in dephosphorylation of downstream targets such as ribosomal protein S6 kinase 1 and 4EBP1 and decreasing cell growth. Disregulation of RTP801 may thus contribute to human tumorigenesis.Synonyms: DDIT-4, DNA-damage-inducible transcript 4, Dig2, HIF-1 responsive protein, Protein regulated in development and DNA damage response 1, REDD-1, REDD1, RTP801

Gene ID: 54541

NCBI Accession: NP_061931

UniProt: Q9NX09

Pathways: Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process