MCM4 antibody (AA 761-863) (AbBy Fluor® 750)

Catalog No. ABIN5006642

This anti-MCM4 antibody is a Rabbit Polyclonal antibody detecting MCM4 in WB, IF (cc) and IF (p). Suitable for Human.

Quick Overview for MCM4 antibody (AA 761-863) (AbBy Fluor® 750) (ABIN5006642)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM4 antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-MCM4 Antibody (AbBy Fluor® 750)

Binding Specificity: AA 761-863

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MCM4

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Alternative Name: MCM4/CDC21

Background:

Synonyms: CDC21, CDC21 homolog, CDC54, DNA replication licensing factor MCM 4, DNA replication licensing factor MCM4, hCdc 21, hCdc21, Homolog of S. pombe cell devision cycle 21, KIAA4003, mcdc21, MCM 4, MCM 4 minichromosome maintenance deficient 4, MCM4, MCM4 minichromosome maintenance deficient 4, MCM4 minichromosome maintenance deficient 4, mitotin S. cerevisiae, MCM4_HUMAN, Mcmd4, MGC33310, Minichromosome Maintenance 4, Minichromosome maintenance complex component 4, Minichromosome maintenance deficient S. cerevisiae 4, Minichromosome maintenance deficient 4, Minichromosome maintenance deficient 4 homolog S. cerevisiae, Minichromosome maintenance, S. cerevisiae, homolog of, 4, mKIAA4003, P1 CDC21, P1-CDC21, P1CDC21, 19G antibody AI325074, AU045576, CDC 21, CDC 54.

Background: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2-6) during apoptosis.

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA