SH3BP4 antibody (C-Term)

Catalog No. ABIN500704

This Rabbit Polyclonal antibody specifically detects SH3BP4 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SH3BP4 antibody (C-Term) (ABIN500704)

Target: SH3BP4 (SH3-Domain Binding Protein 4 (SH3BP4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3BP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SH3BP4 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects SH3BP4.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: SH3BP4 antibody was raised against an 18 amino acid peptide from near the carboxy terminus of human SH3BP4.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for SH3BP4

Target: SH3BP4 (SH3-Domain Binding Protein 4 (SH3BP4))

Alternative Name: SH3BP4

Background: SH3BP4 was initially isolated as a novel cDNA using differential display analysis using cultured human corneal fibroblasts. The protein contains three Asn-Pro-Phe (NPF) motifs, an SH3 domain, a PXXP motif, a bipartite nuclear targeting signal, and a tyrosine phosphorylation site. SH3BP4 has been shown to interact with specific endocytic proteins such as clathrin, dynamin, and the transferrin receptor (TfR) and localizes to TfR-containing coated pits and vesicles. It is thought to be involved in cargo-specific control of clathrin-mediated endocytosis, specifically controlling the internalization of TfR. At least two isoforms of SH3BP4 are known to exist.Synonyms: BOG25, EH-binding protein 10, EHB10, SH3 domain-binding protein 4, SH3BP-4, TTP, Transferrin receptor-trafficking protein

Gene ID: 23677

NCBI Accession: NP_055336

UniProt: Q9P0V3