SIRT2 antibody (N-Term)

Catalog No. ABIN500726

The Rabbit Polyclonal anti-SIRT2 antibody is suitable to detect SIRT2 in samples from Human, Rat and Mouse. It has been validated for WB, IHC (p) and EIA.

Quick Overview for SIRT2 antibody (N-Term) (ABIN500726)

Target: SIRT2 (Sirtuin 2 (SIRT2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SIRT2 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects SIRT2 / SIR2 at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromtography

Immunogen: SIRT2 antibody was raised against a 17 amino acid peptide near the amino terminus of the human SIRT2.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 2.5 - 5 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Alternative Name: SIRT2 / SIR2

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.Synonyms: NAD-dependent deacetylase sirtuin-2, SIR2-like protein 2, SIR2L, SIR2L2, SIRT-2, sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae), sirtuin 2, sirtuin type 2, sirtuin-2

Gene ID: 22933

NCBI Accession: NP_036369

UniProt: Q8IXJ6