SKA1 antibody (C-Term)

Catalog No. ABIN500728

This Rabbit Polyclonal antibody specifically detects SKA1 in WB and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SKA1 antibody (C-Term) (ABIN500728)

Target: SKA1 (Spindle and Kinetochore Associated Complex Subunit 1 (SKA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKA1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SKA1 Antibody

Binding Specificity: C-Term

Specificity: This antibody is specific to SKA1 and will not cross-react with SKA2 or SKA3.

Purification: Peptide Afiinity Chromatography

Immunogen: SKA1 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of Human SKA1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SKA1

Target: SKA1 (Spindle and Kinetochore Associated Complex Subunit 1 (SKA1))

Alternative Name: SKA1

Background: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing.Synonyms: C18orf24, Spindle and kinetochore-associated protein 1

Gene ID: 220134

UniProt: Q96BD8

Pathways: M Phase