SKIP (C-Term) antibody

Catalog No. ABIN500736

Product Details

Target: SKIP

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: This antibody detects SKIP.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: SkiP antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human SkiP.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details

Target: SKIP

Synonyms: 4930544G21Rik antibody, A930009L15Rik antibody, AI852220 antibody, SKIP antibody, mKIAA1678 antibody, RGD1311951 antibody, Skip antibody, SPHK1 interactor, AKAP domain containing antibody, Sphkap antibody, SPHKAP antibody

Background: TGF-beta and the bone morphogenic proteins (BMPs) are key signaling proteins that regulate numerous cellular processes such as embryonic development and tumorigenesis (1). Both signal through the Smad protein family and are negatively regulated by Ski and SnoN, two related proto-oncoproteins (2,3). Ski functions by binding to the Smad proteins activated by TGF-beta and the (BMPs) and preventing their phosphorylation, inhibiting their ability to bind DNA and activate the transcription of downstream genes (4,5). SkiP was originally identified as a Ski-interacting protein (6) and was later found to augment the signals induced by TGF-beta but inhibit transcription induced by BMP-2 (7) in C2C12 cells, suggesting that SkiP is a key player in the signaling cascades inititated by TGF-beta and the BMP protein family.Synonyms: NCoA-62, Nuclear protein SkiP, Nuclear receptor coactivator NCoA-62, SKIIP, SNW domain-containing protein 1, SNW1, Ski-interacting protein

Gene ID: 22938

NCBI Accession: NP_036377

UniProt: Q13573