TREX1 antibody (Center)

Catalog No. ABIN501036

Product Details anti-TREX1 Antibody

Target: TREX1 (three Prime Repair Exonuclease 1 (TREX1))

Binding Specificity: Center

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TREX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects TREX1. It will not cross-react with the related protein TREX2.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Affinity chromatography

Immunogen: TREX1 antibody was raised against a 15 amino acid peptide near the center of human TREX1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for TREX1

Target: TREX1 (three Prime Repair Exonuclease 1 (TREX1))

Alternative Name: TREX1 (TREX1 Products)

Synonyms: AGS1 antibody, CRV antibody, DRN3 antibody, HERNS antibody, 1661 antibody, AU041952 antibody, RGD1309596 antibody, three prime repair exonuclease 1 antibody, CpipJ_CPIJ012074 antibody, Tsp_03749 antibody, TREX1 antibody, Trex1 antibody

Background: Trex1 is the major human 3\' to 5\' exonuclease which is required for checkpoint signaling after DNA damage. It is ubiquitously expressed, binds to single stranded DNA coated with replication protein A that accumulates at sites of DNA damage and recruits the ataxia telangiectasia and Rad3 related protein (ATR), a checkpoint kinase, to sites of DNA damage and replication stress. Trex1 is required for ATR expression. This gene uses two different open reading frames. The upstream ORF encodes proteins which interact with ATR and localize to intranuclear foci induced by DNA damage and are essential components of the DNA damage checkpoint. The downstream ORF encodes proteins with 3\' to 5\' exonuclease activity and may be a subunit of human DNA polymerase III. Multiple transcript variants encoding different isoforms have been found. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, and Cree encephalitis.Synonyms: 3'-5' exonuclease TREX1, DNase III, Three prime repair exonuclease 1

Gene ID: 11277

NCBI Accession: NP_057465

Pathways: Apoptosis