TRIM25 antibody (N-Term)

Catalog No. ABIN501046

This Rabbit Polyclonal antibody specifically detects TRIM25 in WB, IF and EIA. It exhibits reactivity toward Human.

Quick Overview for TRIM25 antibody (N-Term) (ABIN501046)

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM25 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-TRIM25 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects TRIM25 / RNF147 at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Peptide affinity chromatography

Immunogen: TRIM25 antibody was raised against a 15 amino acid peptide from near the amino terminus of human TRIM25.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Alternative Name: TRIM25 / RNF147

Background: TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.Synonyms: EFP, Estrogen-responsive finger protein, RING finger protein 147, Tripartite motif-containing protein 25, ZNF147, Zinc finger protein 147

Gene ID: 7706

NCBI Accession: NP_005073